Gene description for P2RX7
Gene name	purinergic receptor P2X 7
Gene symbol	P2RX7
Other names/aliases	P2X7
Species	Homo sapiens
Experiment description of studies that identified P2RX7 in Melanoma cells
1 Experiment ID 618 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Mass spectrometry [LTQ] PubMed ID 27894104    Organism Homo sapiens Experiment description Proteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers. Authors Hurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr. Journal name Oncotarget Publication year 2016 Sample Melanoma cells Sample name M14 Isolation/purification methods Differential centrifugation Polymer-based precipitation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] EV-TRACK -
2 Experiment ID 619 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Mass spectrometry [LTQ] PubMed ID 27894104    Organism Homo sapiens Experiment description Proteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers. Authors Hurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr. Journal name Oncotarget Publication year 2016 Sample Melanoma cells Sample name MALME-3M Isolation/purification methods Differential centrifugation Polymer-based precipitation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] EV-TRACK -
3 Experiment ID 620 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Mass spectrometry [LTQ] PubMed ID 27894104    Organism Homo sapiens Experiment description Proteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers. Authors Hurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr. Journal name Oncotarget Publication year 2016 Sample Melanoma cells Sample name MDA-MB-435 Isolation/purification methods Differential centrifugation Polymer-based precipitation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] EV-TRACK -
4 Experiment ID 621 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Mass spectrometry [LTQ] PubMed ID 27894104    Organism Homo sapiens Experiment description Proteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers. Authors Hurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr. Journal name Oncotarget Publication year 2016 Sample Melanoma cells Sample name SK-MEL-2 Isolation/purification methods Differential centrifugation Polymer-based precipitation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] EV-TRACK -
5 Experiment ID 3153 Identified molecule protein Extracellular vesicle type Microvesicles MISEV Large EVs Identification method Western blotting PubMed ID 34789738    Organism Homo sapiens Experiment description P2X7 promotes metastatic spreading and triggers release of miRNA-containing exosomes and microvesicles from melanoma cells Authors Pegoraro A, De Marchi E, Ferracin M, Orioli E, Zanoni M, Bassi C, Tesei A, Capece M, Dika E, Negrini M, Di Virgilio F, Adinolfi E. Journal name Cell Death Dis Publication year 2021 Sample Melanoma cells Sample name SK-MEL-28 - 18k pellet Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein miRNA Methods used in the study Western blotting Small RNA sequencing RT-PCR EV-TRACK -
6 Experiment ID 3154 Identified molecule protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Western blotting PubMed ID 34789738    Organism Homo sapiens Experiment description P2X7 promotes metastatic spreading and triggers release of miRNA-containing exosomes and microvesicles from melanoma cells Authors Pegoraro A, De Marchi E, Ferracin M, Orioli E, Zanoni M, Bassi C, Tesei A, Capece M, Dika E, Negrini M, Di Virgilio F, Adinolfi E. Journal name Cell Death Dis Publication year 2021 Sample Melanoma cells Sample name SK-MEL-28 - 100k pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein miRNA Methods used in the study Western blotting Small RNA sequencing RT-PCR EV-TRACK -
7 Experiment ID 3155 Identified molecule protein Extracellular vesicle type Microvesicles MISEV Large EVs Identification method Western blotting PubMed ID 34789738    Organism Homo sapiens Experiment description P2X7 promotes metastatic spreading and triggers release of miRNA-containing exosomes and microvesicles from melanoma cells Authors Pegoraro A, De Marchi E, Ferracin M, Orioli E, Zanoni M, Bassi C, Tesei A, Capece M, Dika E, Negrini M, Di Virgilio F, Adinolfi E. Journal name Cell Death Dis Publication year 2021 Sample Melanoma cells Sample name Ma-Mel-19 - 18k pellet Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein miRNA Methods used in the study Western blotting Small RNA sequencing RT-PCR EV-TRACK -
8 Experiment ID 3156 Identified molecule protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Western blotting PubMed ID 34789738    Organism Homo sapiens Experiment description P2X7 promotes metastatic spreading and triggers release of miRNA-containing exosomes and microvesicles from melanoma cells Authors Pegoraro A, De Marchi E, Ferracin M, Orioli E, Zanoni M, Bassi C, Tesei A, Capece M, Dika E, Negrini M, Di Virgilio F, Adinolfi E. Journal name Cell Death Dis Publication year 2021 Sample Melanoma cells Sample name Ma-Mel-19 - 100k pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein miRNA Methods used in the study Western blotting Small RNA sequencing RT-PCR EV-TRACK -